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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Janelia Fluor 549

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ROBO3
Leu40-Ser545
Accession # Q96MS0

Specificity

Detects ROBO3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 60% cross-reactivity with recombinant mouse ROBO3 is observed, 5% cross-reactivity with recombinant human ROBO2 and recombinant rat ROBO1 is observed, and less than 1% cross‑reactivity with recombinant human ROBO4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for ROBO3 Antibody [Janelia Fluor® 549]

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product AF3076. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: ROBO3

The Robo3 gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, andaxon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLITproteins 1-3, a family of

Long Name

Roundabout Homolog 3

Alternate Names

RIG1

Gene Symbol

ROBO3

Additional ROBO3 Products

Product Documents for ROBO3 Antibody [Janelia Fluor® 549]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ROBO3 Antibody [Janelia Fluor® 549]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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