RNF149 Antibody - Azide and BSA Free

Western Blot: RNF149 Antibody - Azide and BSA Free [NBP2-93343] -

Western Blot: RNF149 Antibody - Azide and BSA Free [NBP2-93343] - Western blot analysis of lysates from Mouse stomach using RNF149 Rabbit pAb at 1:600 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Immunocytochemistry/ Immunofluorescence: RNF149 Antibody - Azide and BSA Free [NBP2-93343] -

Immunocytochemistry/ Immunofluorescence: RNF149 Antibody - Azide and BSA Free [NBP2-93343] - Immunofluorescence analysis of NIH/3T3 cells using RNF149 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western Blot: RNF149 Antibody - Azide and BSA Free [NBP2-93343] -

RNF149 downregulates IRF3 protein level through the proteasome pathway.(A) HEK293T cells were transfected with Flag-IRF3 and a gradient concentration of Myc-RNF149 plasmids for 48 h, and the expression of exogenous IRF3 was detected by Western blot. (B) The expression of endogenous IRF3 protein level in HEK293T cells transfected with a gradient concentration of Myc-RNF149 plasmid for 48 h. (C) HEK293T cells were transfected with shRNF149 and Flag-IRF3 plasmids for 48 h, and the expression of exogenous IRF3 was determined by Western blot. (D) The expression of endogenous IRF3 protein level in HEK293T cells transfected with shRNF149 plasmids for 48 h. (E) The expression of IRF3 in peritoneal macrophages from WT and Rnf149−/− mice was detected by Western blot. (F) HEK293T cells were transfected with vector or Myc-RNF149 plasmid for 48 h, and IRF3 mRNA was detected by RT-qPCR. n=3. Expression levels were normalized to 18S mRNA expression and then to the vector sample. (G) HEK293T cells transfected with Myc-RNF149 and Flag-IRF3 plasmids for 36 h were treated with CHX at different time points, and the protein expression of exogenous IRF3 was determined by Western blot. (H-I) HEK293T cells transfected with Myc-RNF149 and Flag-IRF3 plasmids for 36 h were treated with proteasome inhibitor MG132 or lysosome inhibitor CQ for 12 h, and the protein expression of exogenous IRF3 was determined by Western blot. (F) The P-value was determined using an unpaired t-test. ns, not significant. Data are representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40245000), licensed under a CC-BY license. Not internally tested by Novus Biologicals.