RNF111 Antibody (1C4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # H00054778-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1C4

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RNF111 Antibodies »

Product Specifications

Immunogen

RNF111 (NP_060080, 1 a.a. ~ 108 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSQWTPEYNELYTLKVDMKSEIPSDAPKTQESLKGILLHPEPIGAAKSFPAGVEMINSKVGNEFSHLCDDSQKQEKEMNGNQQEQEKSLVVRKKRKSQQAGPSYVQNC

Specificity

RNF111 - ring finger protein 111

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Novus Biologicals Mouse RNF111 Antibody (1C4) - Azide and BSA Free (H00054778-M05) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-RNF111 Antibody: Cited in 14 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for RNF111 Antibody (1C4) - Azide and BSA Free

Western Blot: RNF111 Antibody (1C4) [H00054778-M05]

Western Blot: RNF111 Antibody (1C4) [H00054778-M05]

Western Blot: RNF111 Antibody (1C4) [H00054778-M05] - Analysis of RNF111 expression in transfected 293T cell line by RNF111 monoclonal antibody (M05), clone 1C4. Lane 1: RNF111 transfected lysatE (107.8 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: RNF111 Antibody (1C4) [H00054778-M05]

Immunocytochemistry/ Immunofluorescence: RNF111 Antibody (1C4) [H00054778-M05]

Immunocytochemistry/Immunofluorescence: RNF111 Antibody (1C4) [H00054778-M05] - Analysis of monoclonal antibody to RNF111 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: RNF111 Antibody (1C4) [H00054778-M05]

Western Blot: RNF111 Antibody (1C4) [H00054778-M05]

Western Blot: RNF111 Antibody (1C4) [H00054778-M05] - Western blot analysis of RNF111 over-expressed 293 cell line, cotransfected with RNF111 Validated Chimera RNAi or non-transfected control. Blot probed with H00054778-M05. GAPDH (36.1 kDa) used as loading control.
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Applications for RNF111 Antibody (1C4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validaton and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RNF111

The protein encoded by this gene contains a RING finger domain, a motif known to be involved in protein-protein and protein-DNA interactions. The mouse counterpart of this gene (Rnf111/arkadia) has been shown to genetically interact with the transforming growth factor (TGF) beta-like factor Nodal, and act as a modulator of the nodal signaling cascade, which is essential for the induction of mesoderm during embryonic development.

Alternate Names

ARK, Arkadia, DKFZp313E0731, DKFZp761D081, E3 ubiquitin-protein ligase Arkadia, EC 6.3.2, EC 6.3.2.-, FLJ38008, ring finger protein 111DKFZp686H1966

Entrez Gene IDs

54778 (Human)

Gene Symbol

RNF111

OMIM

605840 (Human)

UniProt

NP_060080 (Human)

Additional RNF111 Products

Product Documents for RNF111 Antibody (1C4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RNF111 Antibody (1C4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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