RGS17 Antibody - BSA Free

Western Blot: RGS17 Antibody [NBP1-80839]

Western Blot: RGS17 Antibody [NBP1-80839] - Analysis in control (vector only transfected HEK293T lysate) and RGS17 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: RGS17 Antibody [NBP1-80839]

Immunohistochemistry-Paraffin: RGS17 Antibody [NBP1-80839] - Staining of human testis shows strong nuclear positivity in a subset of cells in seminiferus ducts of testis.

Immunocytochemistry/ Immunofluorescence: RGS17 Antibody - BSA Free [NBP1-80839] -

RGS17 tissue-specific knockout scheme and effect of cisplatin in RGS17 expression in whole-mount dissection. (A) Genomic organization of RGS17loxp and RGS17−/− alleles. Exon 2 was flanked with the loxP sequence, which was cleaved using Atoh-1CreEr to generate mice with specific RGS17−/− knockout in the hair cells. (B) The cochleae were harvested from wild-type RGS17+/+ and RGS17−/− mice. Whole-mount dissection for basal turn was immunolabeled with RGS17 antibody to validate RGS17 expression. The expression of RGS17 (green) was located in the outer and inner hair cells of wild-type mice. However, RGS17 was not detected in the outer and inner hair cells of knockout mice. (C) Schematic illustration of the experimental design that describes the dosage and route of administration of control or cisplatin (3.5 mg/kg) for two cycles; each cycle consists of a 4-day cisplatin administration followed by a 10-day recovery period. Mice were sacrificed at the end of the second cycle (D), and cochleae were collected and processed for whole-mount dissection. The basal turns of each cochlea were immunolabeled with RGS17 (green) and DAPI (blue). High levels of RGS17 immunolabeling were detected in the outer and inner hair cells of wild-type RGS17+/+ mice treated with cisplatin. Inducible hair cell-specific RGS17 knockdown (RGS17+/−) mice showed a slight increase of RGS17 immunolabeling in the outer and inner hair cells following cisplatin administration. However, a hair cell-specific knockout of RGS17 abolished the immunolabeling of RGS17 in both the outer and inner hair cells. Figures shown are representative of six independent animals per treatment group. Scale bar = 40 um. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40061942), licensed under a CC-BY license. Not internally tested by Novus Biologicals.