RGNEF Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22282
![Western Blot: RGNEF Antibody [NBP2-22282]](https://resources.bio-techne.com/images/products/RGNEF-Antibody-Western-Blot-NBP2-22282-img0001.jpg "Western Blot: RGNEF Antibody [NBP2-22282]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 1655 and 1705 of human 190 kDa, Rho-Guanine Nucleotide Exchange Factor using the numbering given in entry NP_001171164.1 (GeneID 64283).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit RGNEF Antibody - BSA Free (NBP2-22282) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RGNEF Antibody - BSA Free
![Immunoprecipitation: RGNEF Antibody [NBP2-22282]](https://resources.bio-techne.com/images/products/RGNEF-Antibody-Immunoprecipitation-NBP2-22282-img0003.jpg "Immunoprecipitation: RGNEF Antibody [NBP2-22282]")
Immunoprecipitation: RGNEF Antibody [NBP2-22282]
Immunoprecipitation: RGNEF Antibody [NBP2-22282] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RGNEF antibody NBP2-22282 used for IP at 6 ug/mg lysate. RGNEF was also immunoprecipitated by rabbit anti-RGNEF antibody. For blotting immunoprecipitated RGNEF, NBP2-22282 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for RGNEF Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg
Western Blot
1:1000 - 1:5000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RGNEF
Alternate Names
190 kDa guanine nucleotide exchange factor, p190RHOGEF, Rho-guanine nucleotide exchange factor
Gene Symbol
ARHGEF28
UniProt
Additional RGNEF Products
Product Documents for RGNEF Antibody - BSA Free
Product Specific Notices for RGNEF Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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