Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 112A1021

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RelA/NFkB p65 Antibodies »

Product Specifications

Immunogen

This NfkB p65 antibody was raised against a synthetic peptide corresponding to amino acids 526-539 of human NF-kB (p65) protein.

Reactivity Notes

Predicted to react with Bovine, Gorilla, New World Monkey and Porcine.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for RelA/NFkB p65 Antibody (112A1021) [Biotin]

Western Blot: RelA/NFkB p65 Antibody (112A1021) [Biotin] [NB100-56055] - Analysis ofRelA/NFkB p65 Antibody (112A1021) [Biotin]using NB100-56055 at 2 ug/ml in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.

Applications for RelA/NFkB p65 Antibody (112A1021) [Biotin]

Application
Recommended Usage

Flow Cytometry

1ul/1 million cells

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB100-56055 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RelA/NFkB p65

NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.

Long Name

v-rel Reticuloendotheliosis Viral Oncogene Homolog A

Alternate Names

NFkB p65, NFKB3, p65RelA

Gene Symbol

RELA

Additional RelA/NFkB p65 Products

Product Documents for RelA/NFkB p65 Antibody (112A1021) [Biotin]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RelA/NFkB p65 Antibody (112A1021) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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