RED Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20120
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (97%), Chimpanzee (99%), Mouse (96%), Rat (96%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human RED. The exact sequence is proprietary.
Reactivity Notes
Chicken (83%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit RED Antibody - BSA Free (NBP2-20120) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RED Antibody - BSA Free
![Western Blot: RED Antibody [NBP2-20120]](https://resources.bio-techne.com/images/products/RED-Antibody-Western-Blot-NBP2-20120-img0001.jpg "Western Blot: RED Antibody [NBP2-20120]")
Western Blot: RED Antibody [NBP2-20120]
Western Blot: RED Antibody [NBP2-20120] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HepG2 7. 5% SDS PAGE gel, diluted at 1:3000.
Immunohistochemistry: RED Antibody [NBP2-20120] -
RED antibody detects IK protein at cytosol and nucleus on HeLa xenograft by immunohistochemical analysis.Sample: Paraffin-embedded HeLa xenograft.
RED antibody (NBP2-20120) dilution: 1:500.
br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
Immunocytochemistry/ Immunofluorescence: RED Antibody [NBP2-20120] -
RED antibody detects RED protein at nucleus by immunofluorescent analysis.Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RED protein stained by RED antibody (NBP2-20120) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
Applications for RED Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RED
Alternate Names
chondrosarcoma-associated protein 2, CSA2, Cytokine IK, IK cytokine, down-regulator of HLA II, IK factor, MGC59741, protein Red, Protein RER, RD element, REDprer protein, RER
Gene Symbol
IK
Additional RED Products
Product Documents for RED Antibody - BSA Free
Product Specific Notices for RED Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.