RB1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20127
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Predicted:
Bovine (97%), Porcine (96%), Rat (96%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human RB1. The exact sequence is proprietary.
Reactivity Notes
Chicken (82%).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
106 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit RB1 Antibody - BSA Free (NBP2-20127) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-RB1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RB1 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: RB1 Antibody [NBP2-20127]](https://resources.bio-techne.com/images/products/RB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20127-img0007.jpg "Immunocytochemistry/ Immunofluorescence: RB1 Antibody [NBP2-20127]")
Immunocytochemistry/ Immunofluorescence: RB1 Antibody [NBP2-20127]
Immunocytochemistry/Immunofluorescence: RB1 Antibody [NBP2-20127] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rb stained by Rb antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.![Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127]](https://resources.bio-techne.com/images/products/RB1-Antibody-Immunohistochemistry-Paraffin-NBP2-20127-img0005.jpg "Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127]")
Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127]
Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127] - Human colon carcinoma. Rb antibody dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min![Immunoprecipitation: RB1 Antibody [NBP2-20127]](https://resources.bio-techne.com/images/products/RB1-Antibody-Immunoprecipitation-NBP2-20127-img0004.jpg "Immunoprecipitation: RB1 Antibody [NBP2-20127]")
Immunoprecipitation: RB1 Antibody [NBP2-20127]
Immunoprecipitation: RB1 Antibody [NBP2-20127] - HeLa whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of retinoblastoma 1 protein by 4 ug Rb1 antibody 7.5 % SDS-PAGE The immunoprecipitated retinoblastoma 1 protein was detected by Rb1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Applications for RB1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
Assay dependent
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RB1
Long Name
Retinoblastoma-associated protein
Alternate Names
OSRC, p105-Rb, pp110
Gene Symbol
RB1
Additional RB1 Products
Product Specific Notices for RB1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.