Detects rat PBEF/Visfatin in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant mouse PBEF and recombinant human PBEF is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat PBEF/Visfatin Antibody

Detection of Rat PBEF by Western Blot.

Western blot shows lysates of H4-II-E-C3 rat hepatoma cell line and rat liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Rat PBEF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6384) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PBEF at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Rat PBEF/Visfatin Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: H4‑II‑E‑C3 rat hepatoma cell line and rat liver tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PBEF/Visfatin

PBEF (pre-B cell colony-enhancing factor; also visfatin and nicotinamide phosphoribosyltransferase/Nampt) is a 52‑54 kDa member of the NAPRTase family of molecules. It is a dimer that is found both intracellularly and extracellularly, where it participates in NAD synthesis and induces proinflammatory cytokine synthesis, respectively. PBEF has also been reported to show insulin-like effects on adipocytes by binding to the insulin receptor. Cells known to express PBEF include visceral adipocytes, neutrophils, and capillary endothelium. Rat PBEF is 491 amino acids in length. It contains no signal sequence but is secreted. Potential phosphorylation sites exist at Tyr34, Tyr188 and Ser398. Full‑length (aa 1‑491) rat PBEF shares 99% and 95% aa identity with mouse and human PBEF, respectively.

Long Name

Pre-B-Cell Colony-Enhancing Factor 1

Alternate Names

NAmPRTase, NAMPT, NMPRTase, PBEF1, Visfatin

Entrez Gene IDs

10135 (Human); 59027 (Mouse); 297508 (Rat)

Gene Symbol

NAMPT

UniProt

Q80Z29

Additional PBEF/Visfatin Products

Product Documents for Rat PBEF/Visfatin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Rat PBEF/Visfatin Antibody

For research use only

Citations
Reviews

Rat PBEF/Visfatin Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6384

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity