Nogo-A was detected in immersion fixed frozen sections of rat embryo (E18) using Mouse Anti-Rat Nogo-A Monoclonal Antibody (Catalog # MAB3098) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to spinal cord and dorsal root ganglia. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Rat Nogo-A Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed frozen sections of rat embryo (E18)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nogo-A

Nogo-A, also known as Reticulon-4, is the longest of four splice variants of Nogo. It is a CNS myelin-associated neurite outgrowth inhibitor that is highly expressed in oligodendrocytes. Nogo-A is synthesized as an 1163 amino acid protein and lacks a signal peptide. Within conserved C-terminal reticulon homology domain (RHD), two transmembrane domains, which are separated by a 66 amino acid extracellular loop, exist. Both the N-terminal domain and the 66 amino acid domain (Nogo-66) can be detected on the cell surface and show neurite outgrowth inhibitory activity. The amino acid sequence of rat Nogo-A N-terminal domain is 76% identical to that of human Nogo-A. Within the RHD domain, rat Nogo-A shares 99% and 97% amino acid sequence identity with mouse and human Nogo, respectively.

Long Name

Reticulon 4A

Alternate Names

NI220, NogoA, RTN4, RTN4A

Entrez Gene IDs

57142 (Human); 68585 (Mouse); 83765 (Rat)

Gene Symbol

RTN4

UniProt

Q9JK11

Additional Nogo-A Products

Product Documents for Rat Nogo-A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Rat Nogo-A Antibody

For research use only

Citations
Reviews

Rat Nogo-A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3098

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Rat Nogo-A Antibody

Nogo‑A in Rat Embryo.