Rat MCAM/CD146 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB3250A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 404722
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat MCAM/CD146
Arg19-Lys560
Accession # Q9EPF2
Arg19-Lys560
Accession # Q9EPF2
Specificity
Detects rat MCAM/CD146 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) MCAM, rhBCAM, or rhALCAM is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Rat MCAM/CD146 APC-conjugated Antibody
Detection of MCAM/CD146 in PC‑12 Rat Cell Line by Flow Cytometry.
PC-12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Rat MCAM/CD146 APC-conjugated Monoclonal Antibody (Catalog # FAB3250A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Rat MCAM/CD146 APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: PC‑12 rat adrenal pheochromocytoma cell line
Sample: PC‑12 rat adrenal pheochromocytoma cell line
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: MCAM/CD146
Melanomia Cell Adhesion Molecule (MCAM), also known as CD146 and MUC18, is a putative Ig-superfamily adhesion molecule that is expressed on endothelial cells and a variety of tumor cells. MCAM is associated with tumor progression and metastasis and may be involved in embryonic neural development. An alternately spliced isoform of rat MCAM has a 40 residue deletion in the cytoplasmic domain. Within the extracellular region, rat MCAM shares 72% and 90% amino acid sequence identity with human and mouse MCAM, respectively.
Long Name
Melanoma Cell Adhesion Molecule
Alternate Names
CD146, L-Gicerin, MUC18
Gene Symbol
MCAM
UniProt
Additional MCAM/CD146 Products
Product Documents for Rat MCAM/CD146 APC-conjugated Antibody
Product Specific Notices for Rat MCAM/CD146 APC-conjugated Antibody
For research use only
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