Rat GFR alpha-1/GDNF R alpha-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB560

Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Human, Rat, Rabbit, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Neutralization, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 81401

View all GFR alpha-1/GDNF R alpha-1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat GFR alpha‑1/GDNF R alpha‑1
Asp25-Leu445
Accession # Q62997

Specificity

Detects rat GFR alpha‑1/GDNF R alpha‑1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Ret, recombinant human (rh) GFR alpha-2, rhGFR alpha-3 or rmGFR alpha-2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Rat GFR alpha-1/GDNF R alpha-1 Antibody

Detection of Rat GFRa-1/GDNF Ra-1 antibody by Western Blot.

Detection of Rat GFR alpha‑1/GDNF R alpha‑1 by Western Blot.

Western blot shows lysates of rat spinal cord tissue and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat GFRa-1/GDNF Ra-1 Monoclonal Antibody (Catalog # MAB560) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GFRa-1/GDNF Ra-1 at approximately 60 kDa (as indicated). This experiment was conducted under non-reducing conditions (recommended) and using Immunoblot Buffer Group 1.
GFRa-1/GDNF Ra-1 antibody in Rat Spinal Cord by Immunohistochemistry (IHC-Fr).

GFR alpha‑1/GDNF R alpha‑1 in Rat Spinal Cord.

GFRa-1/GDNF Ra-1 was detected in perfusion fixed frozen sections of rat spinal cord using Rat GFRa-1/GDNF Ra-1 Monoclonal Antibody (Catalog # MAB560) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Rat GFR alpha-1/GDNF R alpha-1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (medulla) and spinal cord

Western Blot

1 µg/mL
Sample: Rat spinal cord tissue and rat kidney tissue under non-reducing condition only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-1/GDNF R alpha-1

Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha- (GFR alpha-1) or GFR alpha-2). GFR alpha-1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha-1 or GFR alpha-2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alphas released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha-1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase. Rat GFR alpha-1 cDNA encodes a 468 amino acid (aa) residue protein with an N‑terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha-1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha-1 shares 93% amino acid identity with rat GFR alpha-1. The expression of the various GFR alphas are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alphas in mediating the activities of the GDNF family of neurotrophic factors.

References

  1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
  2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha 1

Alternate Names

GDNF R alpha-1, GFR alpha1, GFRa-1, GFRA1

Entrez Gene IDs

2674 (Human); 14585 (Mouse); 25454 (Rat)

Gene Symbol

GFRA1

UniProt

Q62997

Additional GFR alpha-1/GDNF R alpha-1 Products

Product Documents for Rat GFR alpha-1/GDNF R alpha-1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Rat GFR alpha-1/GDNF R alpha-1 Antibody

For research use only

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