Detects rat CXCL3/CINC-2 alpha/ beta in ELISAs and Western blots. In sandwich immunoassays, approximately 30% cross‑reactivity with recombinant rat (rr) CINC-3 is observed and less than 0.1% cross‑reactivity with rrCINC-1, recombinant mouse (rm) KC, rmMIP-2, and recombinant human GRO alpha is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat CXCL3/GRO gamma/CINC-2/DCIP-1 Biotinylated Antibody

Rat CXCL3 / GRO gamma / CINC-2 / DCIP-1 ELISA Standard Curve

Recombinant Rat CXCL3/GRO gamma/CINC-2/DCIP-1 (Catalog # 516-CA) was serially diluted and captured by Mouse Anti-Rat CXCL3/GRO gamma/CINC-2/DCIP-1 Monoclonal Antibody (Catalog # MAB516) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat CXCL3/GRO gamma/CINC-2/DCIP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF516) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Rat CXCL3/GRO gamma/CINC-2/DCIP-1 Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Rat CXCL3/CINC-2 alpha (Catalog # 516-CA)
Recombinant Rat CXCL3/CINC-2 beta (Catalog # 517-CB)

Rat CXCL3/CINC-2 alpha/ beta Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Rat CXCL3/CINC-2 alpha/ beta Antibody (Catalog # MAB516)
Standard: Recombinant Rat CXCL3/CINC-2 alpha Protein (Catalog # 516-CA)
Standard: Recombinant Rat CXCL3/CINC-2 beta Protein (Catalog # 517-CB)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL3/GRO gamma/CINC-2/DCIP-1

CINC-2 alpha and -2 beta are mRNA splice variant isoforms that differ by only 3 amino acids at their amino-termini. These proteins are CXC chemokines most closely related to human GROs and mouse MIP-2. They are produced by granulation tissue, activated macrophages and fibroblasts. CINC-2 biological activities are mediated by CXCR1 or CXCR2.

Alternate Names

CINC-2, Dcip1, Gm1960, GRO gamma

Entrez Gene IDs

2921 (Human); 330122 (Mouse); 171551 (Rat)

Gene Symbol

CXCL3

UniProt

Q10746

Additional CXCL3/GRO gamma/CINC-2/DCIP-1 Products

Product Documents for Rat CXCL3/GRO gamma/CINC-2/DCIP-1 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Rat CXCL3/GRO gamma/CINC-2/DCIP-1 Biotinylated Antibody

For research use only

Citations
Reviews

Rat CXCL3/GRO gamma/CINC-2/DCIP-1 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF516

Key Product Details

Species Reactivity

Validated:

Cited:

Applications