Ras Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43784
Key Product Details
Species Reactivity
Human, Mouse, Rat, Goat, Squirrel, Xenopus, Zebrafish
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human KRAS. The exact sequence is proprietary.
Reactivity Notes
Frog (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ras Antibody
![Western Blot: Ras Antibody [NBP2-43784]](https://resources.bio-techne.com/images/products/Ras-Antibody-Western-Blot-NBP2-43784-img0007.jpg "Western Blot: Ras Antibody [NBP2-43784]")
Western Blot: Ras Antibody [NBP2-43784]
Western Blot: Ras Antibody [NBP2-43784] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RAS antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.![Immunohistochemistry-Paraffin: Ras Antibody [NBP2-43784]](https://resources.bio-techne.com/images/products/Ras-Antibody-Immunohistochemistry-Paraffin-NBP2-43784-img0005.jpg "Immunohistochemistry-Paraffin: Ras Antibody [NBP2-43784]")
Immunohistochemistry-Paraffin: Ras Antibody [NBP2-43784]
Immunohistochemistry-Paraffin: Ras Antibody [NBP2-43784] - Rat urinary bladder. Ras antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Ras Antibody [NBP2-43784] -
Western Blot: Ras Antibody [NBP2-43784] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Ras antibody (NBP2-43784) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Applications for Ras Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Ras
The Ras proteins (H-Ras, N-Ras, and two splice variants of K-Ras) define a closely related family of 21 kDa GTPases that cycle between GTP-bound active and GDP-bound inactive states at the cytoplasmic face of the plasma membrane. Receptor tyrosine kinases and G-protein coupled receptors activate Ras, and effectors include Raf and PI 3-kinase. Activating mutations of Ras, which impair GTPase activity and stabilize the active state, are found in nearly one-third of all human cancers.
Long Name
v-Ki-ras2 Kirsten Rat Sarcoma Viral Oncogene Jomolog
Alternate Names
c-K-ras, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, Ki-Ras, KRAS, KRAS1, KRAS2, Oncogene Ras, RASK2
Additional Ras Products
Product Specific Notices for Ras Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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