Ras Antibody (HL1153) - G12A Mutant - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25622
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1153 expressed in HEK293
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding mutant G12A of human K-Ras. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus (93%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ras Antibody (HL1153) - G12A Mutant - Azide and BSA Free
Western Blot: Ras Antibody (HL1153) - G12A Mutant - Azide and BSA Free [NBP3-25622] -
Western Blot: Ras Antibody (HL1153) - G12A Mutant - Azide and BSA Free [NBP3-25622] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12A mutant) antibody [HL1153] (NBP3-25622) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Ras Antibody (HL1153) - G12A Mutant [NBP3-25622] -
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras antibody [HL1153] (NBP3-25622) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Ras Antibody (HL1153) - G12A Mutant - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ras
The Ras proteins (H-Ras, N-Ras, and two splice variants of K-Ras) define a closely related family of 21 kDa GTPases that cycle between GTP-bound active and GDP-bound inactive states at the cytoplasmic face of the plasma membrane. Receptor tyrosine kinases and G-protein coupled receptors activate Ras, and effectors include Raf and PI 3-kinase. Activating mutations of Ras, which impair GTPase activity and stabilize the active state, are found in nearly one-third of all human cancers.
Long Name
v-Ki-ras2 Kirsten Rat Sarcoma Viral Oncogene Jomolog
Alternate Names
c-K-ras, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, Ki-Ras, KRAS, KRAS1, KRAS2, Oncogene Ras, RASK2
Gene Symbol
KRAS
Additional Ras Products
Product Documents for Ras Antibody (HL1153) - G12A Mutant - Azide and BSA Free
Product Specific Notices for Ras Antibody (HL1153) - G12A Mutant - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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