Rap1A/B Antibody (JE35-61)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32893
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE35-61 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human Rap1A/B aa 93-142 / 184. (Uniprot: P62834)
Localization
Cell membrane, Cytoplasm, Cytoplasm, perinuclear region, Cell junction, Early endosome; Cell membrane, Cytoplasm, cytosol, Cell junction.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Rap1A/B Antibody (JE35-61) (NBP3-32893) is a recombinant monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Rap1A/B Antibody (JE35-61)
Western Blot: Rap1A/B Antibody (JE35-61) [NBP3-32893]
Western Blot: Rap1A/B Antibody (JE35-61) [NBP3-32893] - Western blot analysis of Rap1A/B on different lysates with Rabbit anti-Rap1A/B antibody (NBP3-32893) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: HEK-293 cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32893) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Flow Cytometry: Rap1A/B Antibody (JE35-61) [NBP3-32893]
Flow Cytometry: Rap1A/B Antibody (JE35-61) [NBP3-32893] - Flow cytometric analysis of HEK-293 cells labeling Rap1A/B.Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32893, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Applications for Rap1A/B Antibody (JE35-61)
Application
Recommended Usage
Flow Cytometry
1:500-1:1000
Western Blot
1:1000-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Rap1A/B
Ras-related Protein 1A (Rap1A) and Ras-related Protein 1B (Rap1B) are membrane-localized members of the Ras small GTPase superfamily of proteins. Rap1A and Rap1B are 184 amino acid (aa) propeptides that are processed into mature 181 aa proteins each with a predicted molecular mass of 21 kDa. Rap1A appears to play a vital role in the cardiovascular system where it regulates cell attachment, migration, and cell-cell junction formation. Rap1A counteracts the mitogenic functions of Ras and several reports suggest that it mediates the progression of multiple cancers where it may regulate metastasis. Rap1B has also been shown to participate in the organization of the vascular lumen by regulating endothelial cell polarity. It also plays a significant role in platelet function, where it mediates adhesion signaling.
Long Name
Ras-related Protein 1A/B
Alternate Names
RAP1A, RAP1B
Gene Symbol
RAP1A
Additional Rap1A/B Products
Product Documents for Rap1A/B Antibody (JE35-61)
Product Specific Notices for Rap1A/B Antibody (JE35-61)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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