RAD54B Antibody (4A7) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # H00025788-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4A7
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
RAD54B (AAH01965, 801 a.a. ~ 910 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HIQFSVEELKNLFTLHESSDCVTHDLLDCECTGEEVHTGDSLEKFIVSRDCQLGPHHQKSNSLKPLSMSQLKQWKHFSGDHLNLTDPFLERITENVSFIFQNITTQATGT
Reactivity Notes
Human. Other species not tested.
Specificity
RAD54B - RAD54 homolog B (S. cerevisiae)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Novus Biologicals Mouse RAD54B Antibody (4A7) - Azide and BSA Free (H00025788-M01) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RAD54B Antibody (4A7) - Azide and BSA Free
![Western Blot: RAD54B Antibody (4A7) [H00025788-M01]](https://resources.bio-techne.com/images/products/RAD54B-Antibody-4A7-Western-Blot-H00025788-M01-img0007.jpg "Western Blot: RAD54B Antibody (4A7) [H00025788-M01]")
Western Blot: RAD54B Antibody (4A7) [H00025788-M01]
Western Blot: RAD54B Antibody (4A7) [H00025788-M01] - RAD54B monoclonal antibody (M01), clone 4A7 Analysis of RAD54B expression in Hela S3 NE.![Immunocytochemistry/ Immunofluorescence: RAD54B Antibody (4A7) [H00025788-M01]](https://resources.bio-techne.com/images/products/RAD54B-Antibody-4A7-Immunocytochemistry-Immunofluorescence-H00025788-M01-img0005.jpg "Immunocytochemistry/ Immunofluorescence: RAD54B Antibody (4A7) [H00025788-M01]")
Immunocytochemistry/ Immunofluorescence: RAD54B Antibody (4A7) [H00025788-M01]
Immunocytochemistry/Immunofluorescence: RAD54B Antibody (4A7) [H00025788-M01] - Analysis of monoclonal antibody to RAD54B on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: RAD54B Antibody (4A7) [H00025788-M01] - Detection limit for recombinant GST tagged RAD54B is approximately 0.03ng/ml as a capture antibody.
Applications for RAD54B Antibody (4A7) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RAD54B
Alternate Names
DNA repair and recombination protein RAD54B, EC 3.6.1, EC 3.6.4.-, fibrinogen silencer binding protein, FSBP, RAD54 homolog B, RAD54 homolog B (S. cerevisiae), RDH54
Entrez Gene IDs
25788 (Human)
Gene Symbol
RAD54B
OMIM
604289 (Human)
UniProt
Additional RAD54B Products
Product Documents for RAD54B Antibody (4A7) - Azide and BSA Free
Product Specific Notices for RAD54B Antibody (4A7) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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