Rad51D Antibody (5B3/6) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-178
Key Product Details
Species Reactivity
Validated:
Human, Mouse (Negative)
Cited:
Human
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5B3/6
Format
BSA Free
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
His-tagged human Rad51D, overexpressed in E. coli. [UniProt# O75771]
Reactivity Notes
This antibody does not work on mouse.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Rad51D Antibody (5B3/6) - BSA Free
![Western Blot: Rad51D Antibody (5B3/6) [NB100-178]](https://resources.bio-techne.com/images/products/Rad51D-Antibody-5B3-6-Western-Blot-NB100-178-img0003.jpg "Western Blot: Rad51D Antibody (5B3/6) [NB100-178]")
Western Blot: Rad51D Antibody (5B3/6) [NB100-178]
Western Blot: Rad51D Antibody (5B3/6) [NB100-178] - Rad51D detected in HEK293 lysates using a 1:1,000 dilution of NB 100-178 (purified). Photo courtesy of B.T. Bennett & K. Knight of University of Massachusetts Medical School![Immunocytochemistry/ Immunofluorescence: Rad51D Antibody (5B3/6) [NB100-178]](https://resources.bio-techne.com/images/products/Rad51D-Antibody-5B3-6-Immunocytochemistry-Immunofluorescence-NB100-178-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Rad51D Antibody (5B3/6) [NB100-178]")
Immunocytochemistry/ Immunofluorescence: Rad51D Antibody (5B3/6) [NB100-178]
Immunocytochemistry/Immunofluorescence: Rad51D Antibody (5B3/6) [NB100-178] - RAD51D antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).Applications for Rad51D Antibody (5B3/6) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Western Blot
1:1000
Application Notes
This Rad51D (5B3/6) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~40 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Rad51D
Alternate Names
DNA repair protein RAD51 homolog 4, HsTRAD, R51H3Trad, RAD51 homolog D, RAD51DRAD51 (S. cerevisiae)-like 3, RAD51-like 3 (S. cerevisiae), RAD51-like protein 3, recombination repair protein, TRAD
Gene Symbol
RAD51D
Additional Rad51D Products
Product Documents for Rad51D Antibody (5B3/6) - BSA Free
Product Specific Notices for Rad51D Antibody (5B3/6) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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