Rad51 Antibody (5S9B0)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16149
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5S9B0 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Rad51 (Q06609). MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEII
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Rad51 Antibody (5S9B0) (NBP3-16149) is a recombinant monoclonal antibody validated for use in WB and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Rad51 Antibody (5S9B0)
![Western Blot: Rad51 Antibody (5S9B0) [NBP3-16149]](https://resources.bio-techne.com/images/products/Rad51-Antibody-5S9B0-Western-Blot-NBP3-16149-img0001.jpg "Western Blot: Rad51 Antibody (5S9B0) [NBP3-16149]")
Western Blot: Rad51 Antibody (5S9B0) [NBP3-16149]
Western Blot: Rad51 Antibody (5S9B0) [NBP3-16149] - Western blot analysis of extracts of various cell lines, using Rad51 Rabbit mAb (NBP3-16149) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Chromatin Immunoprecipitation: Rad51 Antibody (5S9B0) [NBP3-16149] -
Chromatin Immunoprecipitation: Rad51 Antibody (5S9B0) [NBP3-16149] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using Rad51 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.Applications for Rad51 Antibody (5S9B0)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:50-1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Rad51
Rad51 is involved in both the recombination and repair of DNA damage. Its function may be modulated by interaction with other repair proteins including BRCA1 and BRCA2. Alterations in the Rad51 gene have been associated with increased cancer risk. Rad51 is conserved across species and is homologous to prokaryotic RecA.
Alternate Names
BRCC5, hRAD51, HsRad51, HsT16930, RAD51 (S. cerevisiae) homolog (E coli RecA homolog), RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51 homolog A, RAD51ABRCA1/BRCA2-containing complex, subunit 5, RAD51L3, RecA, E. coli, homolog of, RECADNA repair protein RAD51 homolog 1, RecA-like protein, recombination protein A
Gene Symbol
RAD51
Additional Rad51 Products
Product Documents for Rad51 Antibody (5S9B0)
Product Specific Notices for Rad51 Antibody (5S9B0)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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