Rab5a Antibody (HL1497) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25618

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25618
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Feline

Predicted:

Bovine (93%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1497 expressed in HEK293

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Rab5a Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Rab5a. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Sheep (81%), Chicken (87%), Porcine (81%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Rab5a Antibody (HL1497) - Azide and BSA Free (NBP3-25618) is a recombinant monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Rab5a Antibody (HL1497) - Azide and BSA Free

Rab5a Antibody (HL1497) - Azide and BSA Free

Immunohistochemistry-Paraffin: Rab5a Antibody (HL1497) - Azide and BSA Free [NBP3-25618] -

Immunohistochemistry-Paraffin: Rab5a Antibody (HL1497) - Azide and BSA Free [NBP3-25618] - RAB5A antibody [HL1497] detects RAB5A protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. RAB5A stained by RAB5A antibody [HL1497] (NBP3-25618) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Rab5a Antibody (HL1497) - Azide and BSA Free

Immunohistochemistry-Paraffin: Rab5a Antibody (HL1497) - Azide and BSA Free [NBP3-25618] -

Immunohistochemistry-Paraffin: Rab5a Antibody (HL1497) - Azide and BSA Free [NBP3-25618] - RAB5A antibody [HL1497] detects RAB5A protein by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. RAB5A stained by RAB5A antibody [HL1497] (NBP3-25618) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Rab5a Antibody (HL1497) - Azide and BSA Free

Immunohistochemistry-Paraffin: Rab5a Antibody (HL1497) - Azide and BSA Free [NBP3-25618] -

Immunohistochemistry-Paraffin: Rab5a Antibody (HL1497) - Azide and BSA Free [NBP3-25618] - RAB5A antibody [HL1497] detects RAB5A protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog muscle. RAB5A stained by RAB5A antibody [HL1497] (NBP3-25618) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Applications for Rab5a Antibody (HL1497) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Immunoprecipitation

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rab5a

Rab5 is a 24 kDa GTP-binding protein that regulates the fusion of plasma membrane -derived clathrin-coated vesicles with early endosomes , and homotypic fusion among early endosomes1. Localized to the cytoplasmic side of the plasma membrane, clathrin -coated vesicles and early endosomes, Rab5 appears to regulate vesicle fusion through a cycle of GDP/GTP e xchange and GTP hydrolysis. The different guanine nuc leotide binding states of Rab5 may affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. The GTP-bound, or active, form of Rab5 associates with membrane s and regulates vesicle docking and fusion. Studies using a Rab5 mutant that hydrolyzed xanthosine 5 -triphosphate (XTP) indicated that nucleotide hydrolysis occurs even in the absence of membrane fusion. GTP hydrolysis by Rab5 may determine the frequency of membrane docking and fusion events.

Alternate Names

RAB5A, member RAS oncogene family, RAB5RAS-associated protein RAB5A, ras-related protein Rab-5A

Gene Symbol

RAB5A

Additional Rab5a Products

Product Documents for Rab5a Antibody (HL1497) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Rab5a Antibody (HL1497) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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