RAB35 Antibody - Azide Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20042
![Western Blot: RAB35 Antibody [NBP2-20042]](https://resources.bio-techne.com/images/products/RAB35-Antibody-Western-Blot-NBP2-20042-img0010.jpg "Western Blot: RAB35 Antibody [NBP2-20042]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Hamster
Cited:
Mouse
Predicted:
Bovine (100%), Chicken (98%), Porcine (100%), Rhesus Macaque (100%), Xenopus (96%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human RAB35. The exact sequence is proprietary.
Localization
Cell membrane; Lipid-anchor; Cytoplasmic side (Potential); Melanosome
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit RAB35 Antibody - Azide Free (NBP2-20042) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-RAB35 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RAB35 Antibody - Azide Free
![Immunocytochemistry/ Immunofluorescence: RAB35 Antibody [NBP2-20042]](https://resources.bio-techne.com/images/products/RAB35-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20042-img0011.jpg "Immunocytochemistry/ Immunofluorescence: RAB35 Antibody [NBP2-20042]")
Immunocytochemistry/ Immunofluorescence: RAB35 Antibody [NBP2-20042]
Immunocytochemistry/Immunofluorescence: RAB35 Antibody [NBP2-20042] - RAB35 antibody [N1C3] detects RAB35 protein at Golgi apparatus by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RAB35 stained by RAB35 antibody [N1C3] diluted at 1:500.Blue: Hoechst 33342 staining.![Immunohistochemistry-Paraffin: RAB35 Antibody [NBP2-20042]](https://resources.bio-techne.com/images/products/RAB35-Antibody-Immunohistochemistry-Paraffin-NBP2-20042-img0002.jpg "Immunohistochemistry-Paraffin: RAB35 Antibody [NBP2-20042]")
Immunohistochemistry-Paraffin: RAB35 Antibody [NBP2-20042]
Immunohistochemistry-Paraffin: RAB35 Antibody [NBP2-20042] - Human breast cancer, using RAB35 antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.![Western Blot: RAB35 Antibody [NBP2-20042]](https://resources.bio-techne.com/images/products/RAB35-Antibody-Western-Blot-NBP2-20042-img0009.jpg "Western Blot: RAB35 Antibody [NBP2-20042]")
Western Blot: RAB35 Antibody [NBP2-20042]
Western Blot: RAB35 Antibody [NBP2-20042] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB35 antibody [N1C3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Applications for RAB35 Antibody - Azide Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Format
Azide Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RAB35
Alternate Names
GTP-binding protein RAY, H-ray, RAB1C, RAB35, member RAS oncogene family, Ras-related protein Rab-1C, ras-related protein rab-1c (GTP-binding protein ray), ras-related protein Rab-35, RAY
Gene Symbol
RAB35
UniProt
Additional RAB35 Products
Product Documents for RAB35 Antibody - Azide Free
Product Specific Notices for RAB35 Antibody - Azide Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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