Pyruvate Carboxylase Antibody (10S0V1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16726

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16726-100ul
NBP3-16726-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10S0V1 expressed in HEK293

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Pyruvate Carboxylase/PC Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Pyruvate Carboxylase (P11498). MLKFRTVHGGLRLLGIRRTSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAIYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Pyruvate Carboxylase Antibody (10S0V1) (NBP3-16726) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Pyruvate Carboxylase Antibody (10S0V1)

Western Blot: Pyruvate Carboxylase Antibody (10S0V1) [NBP3-16726]

Western Blot: Pyruvate Carboxylase Antibody (10S0V1) [NBP3-16726]

Western Blot: Pyruvate Carboxylase Antibody (10S0V1) [NBP3-16726] - Western blot analysis of extracts of various cell lines, using Pyruvate Carboxylase Rabbit mAb (NBP3-16726) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for Pyruvate Carboxylase Antibody (10S0V1)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Pyruvate Carboxylase/PC

PCB encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq]

Long Name

Pyruvate Carboxylase

Alternate Names

PC, PCB

Gene Symbol

PC

Additional Pyruvate Carboxylase/PC Products

Product Documents for Pyruvate Carboxylase Antibody (10S0V1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Pyruvate Carboxylase Antibody (10S0V1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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