During my master thesis, I am working on a large scale ribosome affinity purification in Trypanosoma brucei. Unfortunately, we have some problems with the present antibody (IgG against protein A, extracted from chicken). Therefore, we are searching a better and more efficient alternative. Ideally it is extracted from guinea pig, rat, mouse or rabbit. Searching suitable antibodies, I found you have some in your range of products. Since we need to work large scale and will need a bigger amount of antibodies the next months, we would like to get the possibility to test the new antibodies first. Therefore I would like to ask for an aliquot for testing. You would really help me finding an good alternative!

Name: Protein A Antibody (946412) [PerCP]
Brand: Novus Biologicals
SKU: FAB9976C

We unfortunately cannot offer testing samples of our antibodies. I am sorry for any inconvenience. We do have two antibodies to Protein A that meet your host requirements. We fully guarantee our antibodies for all listed species and applications. For any applications and/or species not listed, we offer our Innovators Reward Program. In exchange for a review of your experiment using our product in an untested application or species, we will issue you a credit for the purchase price of the antibody.

Protein A Antibody (946412) [PerCP]

Novus Biologicals, part of Bio-Techne | Catalog # FAB9976C

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

MAB9976

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Allophycocyanin

FAB9976A

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Biotin

FAB9976B

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CoraFluor 1

FAB9976CL1

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DyLight 350

FAB9976D

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DyLight 405

FAB9976E

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DyLight 488

FAB9976K

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DyLight 550

FAB9976L

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DyLight 594

FAB9976M

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DyLight 650

FAB9976W

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DyLight 680

FAB9976Y

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DyLight 755

FAB9976Z

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FITC

FAB9976F

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HRP

FAB9976H

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Janelia Fluor 525

FAB9976JF525

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Janelia Fluor 549

FAB9976I

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Janelia Fluor 646

FAB9976J

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Janelia Fluor 669

FAB9976JF669

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mFluor Violet 450 SE

FAB9976MFV450

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mFluor Violet 500 SE

FAB9976MFV500

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mFluor Violet 610 SE

FAB9976MFV610

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FAB9976P

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Multi-Species

Applications

ELISA

Label

PerCP (Excitation = 490 nm, Emission = 675 nm)

Antibody Source

Monoclonal Rat IgG_2B Clone # 946412

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Protein A Antibodies »

Product Specifications

Immunogen

Recombinant protein A

Specificity

Detects Protein A in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Applications for Protein A Antibody (946412) [PerCP]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Protein A

Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

Alternate Names

PROA, Protein-A

Additional Protein A Products

Product Documents for Protein A Antibody (946412) [PerCP]

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Protein A Antibody (946412) [PerCP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Reviews

Protocols
FAQs

Protein A Antibody (946412) [PerCP]

Novus Biologicals, part of Bio-Techne | Catalog # FAB9976C

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all Protein A Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Applications for Protein A Antibody (946412) [PerCP]

ELISA

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Protein A

Alternate Names

Additional Protein A Products

Product Documents for Protein A Antibody (946412) [PerCP]

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Protein A Antibody (946412) [PerCP]

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