proBDNF Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: proBDNF Antibody - Azide and BSA Free [NB100-98756]

Immunohistochemistry (IHC-P) on paraffin-embedded rat hippocampus sections detecting proBDNF using NB100-98756. The animal was perfused using Osenses Autoperfuser at a pressure of 130 mmHg with 300 mL of 4% formaldehyde, followed by overnight post-fixation prior to paraffin processing and embedding. Heat-induced epitope retrieval (HIER) was performed in Tris-EDTA buffer (pH 9.0) for 20 minutes. Sections were blocked with 0.2% low-fat dry milk (LFDM) in PBST. The primary antibody was applied at a dilution of 1:1000 and incubated for 30 minutes at room temperature. Detection was performed using a HRP Polymer Detection System, followed by visualization with DAB Chromogen. Sections were counterstained with Harris hematoxylin.

Immunohistochemistry-Paraffin: proBDNF Antibody - Azide and BSA Free [NB100-98756]

Immunohistochemistry (IHC-P) on paraffin-embedded rat hippocampus sections detecting proBDNF using NB100-98756. The animal was perfused using at a pressure of 130 mmHg with 300 mL of 4% formaldehyde, followed by overnight post-fixation prior to paraffin processing and embedding. Heat-induced epitope retrieval (HIER) was performed in Tris-EDTA buffer (pH 9.0) for 20 minutes. Sections were blocked with 0.2% low-fat dry milk (LFDM) in PBST. The primary antibody was applied at a dilution of 1:1000 and incubated for 30 minutes at room temperature using the Osenses HistoLogic™ Stainer. Detection was performed using a HRP Polymer Detection System, followed by visualization with DAB Chromogen. Sections were counterstained with Harris hematoxylin.

Immunohistochemistry-Paraffin: proBDNF Antibody - Azide and BSA Free [NB100-98756]

Immunohistochemistry (IHC-P) on paraffin-embedded rat spinal cord sections detecting proBDNF using NB100-98756. The animal was perfused using Osenses Autoperfuser at a pressure of 130 mmHg with 300 mL of 4% formaldehyde, followed by overnight post-fixation prior to paraffin processing and embedding. Heat-induced epitope retrieval (HIER) was performed in Tris-EDTA buffer (pH 9.0) for 20 minutes. Sections were blocked with 0.2% low-fat dry milk (LFDM) in PBST. The primary antibody was applied at a dilution of 1:1000 and incubated for 30 minutes at room temperature. Detection was performed using a HRP Polymer Detection System, followed by visualization with DAB Chromogen. Sections were counterstained with Harris hematoxylin.