PPHLN1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89480

Novus Biologicals, part of Bio-Techne
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NBP1-89480
NBP1-89480-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PPHLN1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KKPPLLDRPGEGSYNRYYSHVDYRDYDEGRSFSHDRRSGPPHRGDESGYRWTRDDHSASRQPEYRDMRDGFRRKSFYSSHYARERSPYKRDNTFFRESPVGRKDSPHSR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (83%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit PPHLN1 Antibody - BSA Free (NBP1-89480) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-PPHLN1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PPHLN1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PPHLN1 Antibody [NBP1-89480]

Immunocytochemistry/ Immunofluorescence: PPHLN1 Antibody [NBP1-89480]

Immunocytochemistry/Immunofluorescence: PPHLN1 Antibody [NBP1-89480] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
PPHLN1 Antibody - BSA Free Immunohistochemistry-Paraffin: PPHLN1 Antibody - BSA Free [NBP1-89480]

Immunohistochemistry-Paraffin: PPHLN1 Antibody - BSA Free [NBP1-89480]

Staining of human cerebral cortex, colon, liver and testis using Anti-PPHLN1 antibody NBP1-89480 (A) shows similar protein distribution across tissues to independent antibody NBP2-38431 (B).
PPHLN1 Antibody - BSA Free Immunohistochemistry-Paraffin: PPHLN1 Antibody - BSA Free [NBP1-89480]

Immunohistochemistry-Paraffin: PPHLN1 Antibody - BSA Free [NBP1-89480]

Analysis in human testis and pancreas tissues using NBP1-89480 antibody. Corresponding PPHLN1 RNA-seq data are presented for the same tissues.
View 6 more images

Applications for PPHLN1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. PPHLN1 antibody validated for WB from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using NBP1-89480 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPHLN1

PPHLN1 is encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Alternate Names

Gastric cancer antigen Ga50, HSPC232, MGC48786, periphilin 1, periphilin-1

Gene Symbol

PPHLN1

Additional PPHLN1 Products

Product Documents for PPHLN1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PPHLN1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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