Please differentiate to me between PPAR and PGC clearly. I am confused with the difference between these two

Name: PPAR alpha/NR1C1 [p Ser12] Antibody
Brand: Novus Biologicals
SKU: NB120-3484

Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.

PPAR alpha/NR1C1 [p Ser12] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-3484

Submit a review

Citations (1)

Product Datasheet / COA / SDS

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all PPAR alpha/NR1C1 Antibodies »

Product Specifications

Immunogen

Synthetic phosphopeptide corresponding to residues I(8) C P L (pS) P L E A D D L(19) of mouse PPAR alpha.

Modification

p Ser12

Localization

Nuclear

Specificity

NB120-3484 detects phospho-peroxisome proliferator activated receptor (PPAR) alpha S12 from mouse tissues.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPAR alpha/NR1C1 [p Ser12] Antibody

Western Blot: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484]

Western Blot: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of 25 ug of C2C12 (lane 1), NIH-3T3 (lane 2), 3T3-L1 (lane 3) and HepG2 (lane 4) cell lysates onto an SDS polyacrylamide gel.

Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484]

Immunocytochemistry/Immunofluorescence: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left).

Flow Cytometry: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484] - Analysis of PPAR alpha [pSer12] was done on MCF7 cells.

View 1 more images

Applications for PPAR alpha/NR1C1 [p Ser12] Antibody

Application

Recommended Usage

Flow Cytometry

3 - 5 ug / 10^6 cells

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Western Blot

1:100 - 1:1000

Application Notes

WB: Detects an approx. 52 kDa protein which corresponds to phospho-PPAR alpha S12 from mouse adipose tissue extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PPAR alpha/NR1C1

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex. The corresponding gene for the phospho-PPAR alpha is NR1C1.

Long Name

Peroxisome Proliferator-activated Receptor alpha

Alternate Names

NR1C1, PPARA

Entrez Gene IDs

19013 (Mouse)

Gene Symbol

PPARA

Additional PPAR alpha/NR1C1 Products

Product Documents for PPAR alpha/NR1C1 [p Ser12] Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for PPAR alpha/NR1C1 [p Ser12] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Protocols
FAQs

PPAR alpha/NR1C1 [p Ser12] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-3484

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all PPAR alpha/NR1C1 Antibodies »

Product Specifications

Immunogen

Modification

Localization

Specificity

Clonality

Host

Isotype

Scientific Data Images for PPAR alpha/NR1C1 [p Ser12] Antibody

Western Blot: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484]

Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484]

Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 [p Ser12] Antibody [NB120-3484]

Applications for PPAR alpha/NR1C1 [p Ser12] Antibody

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: PPAR alpha/NR1C1

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

Additional PPAR alpha/NR1C1 Products

Product Documents for PPAR alpha/NR1C1 [p Ser12] Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for PPAR alpha/NR1C1 [p Ser12] Antibody