POU4F3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88349
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Rat (99%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation
Cited:
Immunohistochemistry-Paraffin, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: HQGLEGDLLEHISPTLSVSGLGAPEHSVMPAQIHPHHLGAMGHLHQAMGMSHPHTVAPHSAMPACLSDVESDPR
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:28402854).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit POU4F3 Antibody - BSA Free (NBP1-88349) is a polyclonal antibody validated for use in IHC, ICC/IF and ChIP. Anti-POU4F3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for POU4F3 Antibody - BSA Free
![POU4F3 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: POU4F3 Antibody - BSA Free [NBP1-88349]](https://resources.bio-techne.com/images/products/nbp1-88349_rabbit-polyclonal-pou4f3-antibody-6520251620500.jpg "Immunocytochemistry/ Immunofluorescence: POU4F3 Antibody - BSA Free [NBP1-88349]")
Immunocytochemistry/ Immunofluorescence: POU4F3 Antibody - BSA Free [NBP1-88349]
Staining of human cell line U-2 OS shows localization to nucleoplasm.![POU4F3 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: POU4F3 Antibody - BSA Free [NBP1-88349]](https://resources.bio-techne.com/images/products/nbp1-88349_rabbit-polyclonal-pou4f3-antibody-25620259103174.jpg "Chromatin Immunoprecipitation-exo-Seq: POU4F3 Antibody - BSA Free [NBP1-88349]")
Chromatin Immunoprecipitation-exo-Seq: POU4F3 Antibody - BSA Free [NBP1-88349]
ChIP-Exo-Seq composite graph for Anti-POU4F3 (NBP1-88349) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for POU4F3 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation
1-10µg per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
Reported in (PMID: 28402854)
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: POU4F3
Alternate Names
Brain-3C, Brain-specific homeobox/POU domain protein 3C, BRN3.1, Brn-3C, BRN3Cbrn-3C, DFNA15, MGC138412, POU class 4 homeobox 3, POU domain class 4, transcription factor 3, POU domain, class 4, transcription factor 3
Gene Symbol
POU4F3
Additional POU4F3 Products
Product Documents for POU4F3 Antibody - BSA Free
Product Specific Notices for POU4F3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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