POU3F2/OCT7 Antibody (CL6232) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43884

Novus Biologicals, part of Bio-Techne
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NBP3-43884
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Catalog #
BSA Free
NBP2-61437
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL6232

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all POU3F2 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P20265, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse POU3F2/OCT7 Antibody (CL6232) - Azide and BSA Free (NBP2-61437) is a monoclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for POU3F2/OCT7 Antibody (CL6232) - Azide and BSA Free

POU3F2/OCT7 Antibody (CL6232) - Azide and BSA Free Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP3-43884]

Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP3-43884]

Staining of mouse brain shows moderate to strong nuclear positivity in neurons in layers 2-5 of cerebral cortex.
POU3F2/OCT7 Antibody (CL6232) - Azide and BSA Free Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP3-43884]

Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP3-43884]

Staining of rat cerebral cortex shows moderate to strong nuclear positivity in neurons in layers 3-4.
POU3F2/OCT7 Antibody (CL6232) - Azide and BSA Free Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP3-43884]

Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP3-43884]

Staining of mouse brain shows strong nuclear positivity in neurons in the lateral ventricle wall.
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Applications for POU3F2/OCT7 Antibody (CL6232) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POU3F2

POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM]

Long Name

POU Class 3 Homeobox 2

Alternate Names

Brain-2, BRN2, Oct7, OTF7

Gene Symbol

POU3F2

Additional POU3F2 Products

Product Documents for POU3F2/OCT7 Antibody (CL6232) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POU3F2/OCT7 Antibody (CL6232) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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