POU3F2/OCT7 Antibody (CL6228) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43883

Novus Biologicals, part of Bio-Techne
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NBP3-43883
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BSA Free
NBP2-61436
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL6228

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all POU3F2 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P20265, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse POU3F2/OCT7 Antibody (CL6228) - Azide and BSA Free (NBP2-61436) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for POU3F2/OCT7 Antibody (CL6228) - Azide and BSA Free

POU3F2/OCT7 Antibody (CL6228) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: POU3F2/OCT7 Antibody [NBP3-43883]

Immunocytochemistry/ Immunofluorescence: POU3F2/OCT7 Antibody [NBP3-43883]

Staining of U-251 cells using the Anti-POU3F2/OCT7 monoclonal antibody) .
POU3F2/OCT7 Antibody (CL6228) - Azide and BSA Free Western Blot: POU3F2/OCT7 Antibody [NBP3-43883]

Western Blot: POU3F2/OCT7 Antibody [NBP3-43883]

Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-POU3F2/OCT7 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
POU3F2/OCT7 Antibody (CL6228) - Azide and BSA Free Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP3-43883]

Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP3-43883]

Staining of mouse brain shows moderate nuclear positivity in neurons in cortical layers 2-4.
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Applications for POU3F2/OCT7 Antibody (CL6228) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF, Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POU3F2

POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM]

Long Name

POU Class 3 Homeobox 2

Alternate Names

Brain-2, BRN2, Oct7, OTF7

Gene Symbol

POU3F2

Additional POU3F2 Products

Product Documents for POU3F2/OCT7 Antibody (CL6228) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POU3F2/OCT7 Antibody (CL6228) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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