Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36523
Key Product Details
Species Reactivity
Non-species specific
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # HWA4C4
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic peptide containing the ubiquitin K63-isopeptide motif.
Reactivity Notes
Does not cross-react with any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitinylated species.
Specificity
Antibody specifically recognizes K63-linked polyubiquitin chains, but not any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitin chain or monoubiquitin. The specificity of this K63-linked ubiquitin specific monoclonal antibody was demonstrated using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in both cell lysates and intact cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
Novus Biologicals Mouse Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage - BSA Free (NBP2-36523) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage - BSA Free
![Western Blot: Poly-Ubiquitin Antibody (HWA4C4)K63 Linkage [NBP2-36523]](https://resources.bio-techne.com/images/products/Poly-Ubiquitin-K63-Linkage-Antibody-HWA4C4-Western-Blot-NBP2-36523-img0019.jpg "Western Blot: Poly-Ubiquitin Antibody (HWA4C4)K63 Linkage [NBP2-36523]")
Western Blot: Poly-Ubiquitin Antibody (HWA4C4)K63 Linkage [NBP2-36523]
Western Blot: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523] - Poly-Ubiquitin K63 Linkage Antibody (HWA4C4) [NBP2-36523] - Analysis of HeLa S100 Fraction. (Lane A) and MAb to Polyubiquitinylated Conjugates (clone FK1) (Lane B) at 1:1000 dilution.![Immunohistochemistry-Paraffin: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523]](https://resources.bio-techne.com/images/products/Poly-Ubiquitin-K63-Linkage-Antibody-HWA4C4-Immunohistochemistry-Paraffin-NBP2-36523-img0017.jpg "Immunohistochemistry-Paraffin: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523]")
Immunohistochemistry-Paraffin: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523]
Immunohistochemistry-Paraffin: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523] - Poly-Ubiquitin K63 Linkage Antibody (HWA4C4) [NBP2-36523] - Sections of hippocampus from human Alzheimers disease brain stained at 1:100 dilution with biotinylated secondary antibody and DAB detection.![Western Blot: Poly-Ubiquitin Antibody (HWA4C4)K63 Linkage [NBP2-36523]](https://resources.bio-techne.com/images/products/Poly-Ubiquitin-K63-Linkage-Antibody-HWA4C4-Western-Blot-NBP2-36523-img0018.jpg "Western Blot: Poly-Ubiquitin Antibody (HWA4C4)K63 Linkage [NBP2-36523]")
Western Blot: Poly-Ubiquitin Antibody (HWA4C4)K63 Linkage [NBP2-36523]
Western Blot: Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage [NBP2-36523] - Poly-Ubiquitin K63 Linkage Antibody (HWA4C4) [NBP2-36523] - Analysis at 1:1000 dilutionApplications for Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:100
Immunohistochemistry-Paraffin
1:100
Western Blot
1:500 - 1:1000
Application Notes
Specificity is temperature sensitive. Western blot membranes must not be autoclaved. Excessive heat should be avoided in all applications.K63-linked polyubiquitin modified proteins comprise only a proportion of total cellular polyubiquitinylated species. Increased sample loading may therefore be required for analysis by Western blot. Do not use milk as blocking solution. Use 1% BSA in an appropriate buffer instead.
Formulation, Preparation, and Storage
Purification
Ion exchange chromatography
Formulation
PBS
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Poly-Ubiquitin
Poly-Ubiquitin chains are composed of Ubiquitin monomers that are covalently linked through isopeptide bonds, which typically form between a lysine residue of one Ubiquitin molecule and the C-terminal glycine residue of another Ubiquitin molecule. Each human Ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse Ubiquitin, respectively. Seven of the 76 aa in Ubiquitin are lysine residues that can participate in poly-Ubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes.
Alternate Names
PolyUbiquitin
Gene Symbol
RPS27A
Additional Poly-Ubiquitin Products
Product Documents for Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage - BSA Free
Product Specific Notices for Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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