Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16232
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5Y8J0 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Poly-Ubiquitin (P0CG47). MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage (NBP3-16232) is a recombinant monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage
Western Blot: Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage [NBP3-16232] -
Western Blot: Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage [NBP3-16232] - Western blot analysis of lysates from HeLa cells using Poly-Ubiquitin Rabbit mAb at 1:1000 dilution incubated overnight at 4C. HeLa cells were treated by MG132 (50 uM) at 37C for 90 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.
Immunocytochemistry/ Immunofluorescence: Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage [NBP3-16232] -
Immunocytochemistry/ Immunofluorescence: Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage [NBP3-16232] - Confocal imaging of MCF7 cells using Poly-Ubiquitin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage [NBP3-16232] -
Confocal imaging of HeLa cells (treated with MG-132) and HeLa cells (untreated) cells using K48-linkage Specific Ubiquitin Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.Applications for Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Poly-Ubiquitin
Poly-Ubiquitin chains are composed of Ubiquitin monomers that are covalently linked through isopeptide bonds, which typically form between a lysine residue of one Ubiquitin molecule and the C-terminal glycine residue of another Ubiquitin molecule. Each human Ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse Ubiquitin, respectively. Seven of the 76 aa in Ubiquitin are lysine residues that can participate in poly-Ubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes.
Alternate Names
PolyUbiquitin
Gene Symbol
RPS27A
Additional Poly-Ubiquitin Products
Product Documents for Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage
Product Specific Notices for Poly-Ubiquitin Antibody (5Y8J0) - K48 Linkage
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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