Podocin/NPHS2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18129
![Immunocytochemistry/ Immunofluorescence: Podocin/NPHS2 Antibody [NBP3-18129]](https://resources.bio-techne.com/images/products/Podocin-NPHS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18129-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Podocin/NPHS2 Antibody [NBP3-18129]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Partial recombinant protein made to an internal portion of human Podocin/NPHS2 (between amino acids 250-400) [UniProt Q9NP85].
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Podocin/NPHS2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: Podocin/NPHS2 Antibody [NBP3-18129]
Immunocytochemistry/Immunofluorescence: Podocin/NPHS2 Antibody [NBP3-18129] - RCC4 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Podocin/NPHS2 Antibody (NBP3-18129) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody [NBP3-18129]](https://resources.bio-techne.com/images/products/Podocin-NPHS2-Antibody-Immunohistochemistry-Paraffin-NBP3-18129-img0001.jpg "Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody [NBP3-18129]")
Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody [NBP3-18129]
Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody [NBP3-18129] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of Podocin/NPHS2 antibody (NBP3-18129). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.Applications for Podocin/NPHS2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1 ug/ml
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Western Blot
1 - 2 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Podocin/NPHS2
Alternate Names
Nephrosis 2, NPHS2, PDCN, SRN1
Gene Symbol
NPHS2
Additional Podocin/NPHS2 Products
Product Documents for Podocin/NPHS2 Antibody - BSA Free
Product Specific Notices for Podocin/NPHS2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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