PKA RI beta Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87451
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QQVLKDCIVHLCISKPERPMKFLREHFEKLEKEENRQILARQKSNSQSDSHDEEVSPT
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit PKA RI beta Antibody - BSA Free (NBP1-87451) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PKA RI beta Antibody - BSA Free
![Western Blot: PKA RI beta Antibody [NBP1-87451]](https://resources.bio-techne.com/images/products/PKA-regulatory-subunit-I-beta-Antibody-Western-Blot-NBP1-87451-img0007.jpg "Western Blot: PKA RI beta Antibody [NBP1-87451]")
Western Blot: PKA RI beta Antibody [NBP1-87451]
Western Blot: PKA RI beta Antibody [NBP1-87451] - Analysis in control (vector only transfected HEK293T lysate) and PRKAR1B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunohistochemistry-Paraffin: PKA RI beta Antibody [NBP1-87451]](https://resources.bio-techne.com/images/products/PKA-regulatory-subunit-I-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-87451-img0006.jpg "Immunohistochemistry-Paraffin: PKA RI beta Antibody [NBP1-87451]")
Immunohistochemistry-Paraffin: PKA RI beta Antibody [NBP1-87451]
Immunohistochemistry-Paraffin: PKA RI beta Antibody [NBP1-87451] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.Applications for PKA RI beta Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PKA RI beta
The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription. Catalytic PKA subunit isoforms, Cα, Cβ and, in humans, Cγ, have been identified.
Long Name
cAMP-dependent Protein Kinase Regulatory Type I beta
Alternate Names
PRKAR1B, RIbeta
Gene Symbol
PRKAR1B
Additional PKA RI beta Products
Product Documents for PKA RI beta Antibody - BSA Free
Product Specific Notices for PKA RI beta Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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