PKA RI beta Antibody (OTI2A3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73430

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2A3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PKA RI beta Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PRKAR1B (NP_002726) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse PKA RI beta Antibody (OTI2A3) - Azide and BSA Free (NBP2-00634) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PKA RI beta Antibody (OTI2A3) - Azide and BSA Free

Western Blot: PKA RI beta Antibody (OTI2A3)Azide and BSA Free [NBP2-73430]

Western Blot: PKA RI beta Antibody (OTI2A3)Azide and BSA Free [NBP2-73430]

Western Blot: PKA RI beta Antibody (OTI2A3) - Azide and BSA Free [NBP2-73430] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKA regulatory subunit I beta (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted wi
Immunocytochemistry/ Immunofluorescence: PKA RI beta Antibody (OTI2A3) - Azide and BSA Free [NBP2-73430]

Immunocytochemistry/ Immunofluorescence: PKA RI beta Antibody (OTI2A3) - Azide and BSA Free [NBP2-73430]

Immunocytochemistry/Immunofluorescence: PKA RI beta Antibody (OTI2A3) - Azide and BSA Free [NBP2-73430] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PKA regulatory subunit I beta.
Immunohistochemistry: PKA RI beta Antibody (OTI2A3) - Azide and BSA Free [NBP2-73430]

Immunohistochemistry: PKA RI beta Antibody (OTI2A3) - Azide and BSA Free [NBP2-73430]

Immunohistochemistry: PKA RI beta Antibody (OTI2A3) - Azide and BSA Free [NBP2-73430] - Staining of paraffin-embedded Human lymph node tissue using anti-PKA regulatory subunit I beta mouse monoclonal antibody.
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Applications for PKA RI beta Antibody (OTI2A3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKA RI beta

The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription. Catalytic PKA subunit isoforms, Cα, Cβ and, in humans, Cγ, have been identified.

Long Name

cAMP-dependent Protein Kinase Regulatory Type I beta

Alternate Names

PRKAR1B, RIbeta

Gene Symbol

PRKAR1B

Additional PKA RI beta Products

Product Documents for PKA RI beta Antibody (OTI2A3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKA RI beta Antibody (OTI2A3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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