PKA 2 beta Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33773

Novus Biologicals, part of Bio-Techne
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NBP1-33773
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKA 2 beta Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKA 2 beta. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (84%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit PKA 2 beta Antibody - BSA Free (NBP1-33773) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PKA 2 beta Antibody - BSA Free

Western Blot: PKA 2 beta Antibody [NBP1-33773]

Western Blot: PKA 2 beta Antibody [NBP1-33773]

Western Blot: PKA 2 beta Antibody [NBP1-33773] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKA 2 beta antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: PKA 2 beta Antibody [NBP1-33773]

Immunocytochemistry/ Immunofluorescence: PKA 2 beta Antibody [NBP1-33773]

Immunocytochemistry/Immunofluorescence: PKA 2 beta Antibody [NBP1-33773] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: PKA 2 beta Antibody [NBP1-33773]

Western Blot: PKA 2 beta Antibody [NBP1-33773]

Western Blot: PKA 2 beta Antibody [NBP1-33773] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.
View 2 more images

Applications for PKA 2 beta Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PKA 2 beta

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.

Long Name

cAMP-dependent protein kinase type II-beta regulatory subunit

Alternate Names

PRKAR2B

Gene Symbol

PRKAR2B

Additional PKA 2 beta Products

Product Documents for PKA 2 beta Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKA 2 beta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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