PIK3CA Antibody (23GB950)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50939
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB950
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human PIK3CA (Uniprot: P42336)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PIK3CA Antibody (23GB950)
Immunocytochemistry/ Immunofluorescence: PIK3CA Antibody (23GB950)[NBP3-50939] -
Immunocytochemical staining of HepG2 cells with PIK3CA antibody (1:1,000) . Nuclei were stained blue with DAPI; PIK3CA was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Flow Cytometry: PIK3CA Antibody (23GB950)[NBP3-50939] -
Flow cytometric analysis of PIK3CAexpression in H9c2 cells using PIK3CA antibody (1:2,000). Green, isotype control; red, PI3 kinase p110 alpha.
Western Blot: PIK3CA Antibody (23GB950)[NBP3-50939] -
Western blotting analysis using PIK3CA antibody (1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:50,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.Applications for PIK3CA Antibody (23GB950)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PIK3CA
Long Name
PI3-Kinase P110 Subunit Alpha
Alternate Names
CLAPO, CLOVE, CWS5, MCMTC, P110alpha, PI3Kalpha
Gene Symbol
PIK3CA
Additional PIK3CA Products
Product Documents for PIK3CA Antibody (23GB950)
Product Specific Notices for PIK3CA Antibody (23GB950)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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