PHGDH Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87311
![Western Blot: PHGDH Antibody [NBP1-87311]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-Western-Blot-NBP1-87311-img0015.jpg "Western Blot: PHGDH Antibody [NBP1-87311]")
![Western Blot: PHGDH Antibody [NBP1-87311]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-Western-Blot-NBP1-87311-img0018.jpg "Western Blot: PHGDH Antibody [NBP1-87311]")
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LEEIWPLCDFITVHTPLLPSTTGLLNDNTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQFVDM
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit PHGDH Antibody - BSA Free (NBP1-87311) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-PHGDH Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PHGDH Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP1-87311]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87311-img0016.jpg "Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP1-87311]")
Immunocytochemistry/ Immunofluorescence: PHGDH Antibody [NBP1-87311]
Immunocytochemistry/Immunofluorescence: PHGDH Antibody [NBP1-87311] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-87311-img0013.jpg "Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311]")
Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311]
Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311] - Immunohistochemical staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal and glial cells.![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-87311-img0010.jpg "Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311]")
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-87311-img0011.jpg "Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311]")
![Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-Immunohistochemistry-Paraffin-NBP1-87311-img0012.jpg "Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311]")
![Simple Western: PHGDH Antibody [NBP1-87311]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-Simple-Western-NBP1-87311-img0007.jpg "Simple Western: PHGDH Antibody [NBP1-87311]")
![Simple Western: PHGDH Antibody [NBP1-87311]](https://resources.bio-techne.com/images/products/PHGDH-Antibody-Simple-Western-NBP1-87311-img0008.jpg "Simple Western: PHGDH Antibody [NBP1-87311]")
Applications for PHGDH Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Simple Western
1:20
Western Blot
0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC-Paraffin HIER pH6 retrieval is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 56 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 56 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PHGDH
Long Name
3-Phosphoglycerate Dehydrogenase
Alternate Names
3PGDH, PDGH3
Gene Symbol
PHGDH
Additional PHGDH Products
Product Documents for PHGDH Antibody - BSA Free
Product Specific Notices for PHGDH Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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