PHF8 Antibody (24GB945)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50576

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NBP3-50576-100ul
NBP3-50576-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB945

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PHF8 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human PHF8 (Uniprot: Q9UPP1)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHF8 Antibody (24GB945)

PHF8 Antibody (24GB945)

Immunocytochemistry/ Immunofluorescence: PHF8 Antibody (24GB945)[NBP3-50576] -

Immunocytochemical staining of HepG2 cells with PHF8 antibody (1:1,000). Nuclei were stained blue with DAPI; PHF8 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
PHF8 Antibody (24GB945)

Flow Cytometry: PHF8 Antibody (24GB945)[NBP3-50576] -

Flow cytometric analysis of PHF8expression in HepG2 cells using PHF8 antibody (1:2,000). Green, isotype control; red, PHD finger protein 8.
PHF8 Antibody (24GB945)

Western Blot: PHF8 Antibody (24GB945)[NBP3-50576] -

Western blotting analysis using PHF8 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for PHF8 Antibody (24GB945)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PHF8

PHF8 (PHD finger protein 8) is a member of the jumonji family of proteins and contains a jumonji C (JmjC) domain. The JmjC proteins are predicted to be metalloenzymes that play a role a chromatin remodeling and histone demethylation. PHF8 also bears a PHD (plant homeodomain)- type zinc-finger, a domain also found to be involved in chromatin remodeling and transcriptional regulation. Truncating mutations in PHF8 are associated with X-linked mental retardation and cleft lip/cleft palate. PHF8 is also known as ZNF422 and KIAA1111.

Long Name

Histone lysine demethylase PHF8

Alternate Names

KIAA1111, ZNF422

Gene Symbol

PHF8

Additional PHF8 Products

Product Documents for PHF8 Antibody (24GB945)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PHF8 Antibody (24GB945)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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