PD-1 Antibody (4F12) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-04328
![Western Blot: PD-1 Antibody (4F12)BSA Free [NBP1-04328]](https://resources.bio-techne.com/images/products/PD-1-Antibody-4F12-Western-Blot-NBP1-04328-img0005.jpg "Western Blot: PD-1 Antibody (4F12)BSA Free [NBP1-04328]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Canine
Cited:
Canine
Applications
Validated:
Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 4F12
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human PD-1 (21-167aa) purified from E. coli
Reactivity Notes
Use in Canine reported in scientific literature (PMID:34923192) .
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for PD-1 Antibody (4F12) - BSA Free
Western Blot: PD-1 Antibody (4F12)BSA Free [NBP1-04328]
Western Blot: PD-1 Antibody (4F12) [NBP1-04328] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCD1 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate, Lane 2.: Raji cell lysate![Immunocytochemistry/ Immunofluorescence: PD-1 Antibody (4F12) - BSA Free [NBP1-04328]](https://resources.bio-techne.com/images/products/PD-1-Antibody-4F12-Immunocytochemistry-Immunofluorescence-NBP1-04328-img0004.jpg "Immunocytochemistry/ Immunofluorescence: PD-1 Antibody (4F12) - BSA Free [NBP1-04328]")
Immunocytochemistry/ Immunofluorescence: PD-1 Antibody (4F12) - BSA Free [NBP1-04328]
Immunocytochemistry/Immunofluorescence: PD-1 Antibody (4F12) [NBP1-04328] - HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PDCD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).![Flow Cytometry: PD-1 Antibody (4F12) - BSA Free [NBP1-04328]](https://resources.bio-techne.com/images/products/PD-1-Antibody-4F12-Flow-Cytometry-NBP1-04328-img0003.jpg "Flow Cytometry: PD-1 Antibody (4F12) - BSA Free [NBP1-04328]")
Flow Cytometry: PD-1 Antibody (4F12) - BSA Free [NBP1-04328]
Flow Cytometry: PD-1 Antibody (4F12) [NBP1-04328] - Flow cytometry analysis of PDCD1 in HeLa cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.Applications for PD-1 Antibody (4F12) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
2-5ug
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PD-1
Long Name
Programmed Death-1
Alternate Names
CD279, PD1, PDCD1, SLEB2
Gene Symbol
PDCD1
UniProt
Additional PD-1 Products
Product Documents for PD-1 Antibody (4F12) - BSA Free
Product Specific Notices for PD-1 Antibody (4F12) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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