PCNA associated factor Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32117
![Immunohistochemistry-Paraffin: PCNA associated factor Antibody [NBP2-32117]](https://resources.bio-techne.com/images/products/PCNA-associated-factor-Antibody-Immunohistochemistry-NBP2-32117-img0002.jpg "Immunohistochemistry-Paraffin: PCNA associated factor Antibody [NBP2-32117]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 1 to 50 of human PCNA-Associated Factor using the numbering given in entry NP_055551.1 (GeneID 9768).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit PCNA associated factor Antibody - BSA Free (NBP2-32117) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PCNA associated factor Antibody - BSA Free
Immunohistochemistry-Paraffin: PCNA associated factor Antibody [NBP2-32117]
Immunohistochemistry-Paraffin: PCNA associated factor Antibody [NBP2-32117] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PAF used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).![Immunoprecipitation: PCNA associated factor Antibody [NBP2-32117]](https://resources.bio-techne.com/images/products/PCNA-associated-factor-Antibody-Immunoprecipitation-NBP2-32117-img0001.jpg "Immunoprecipitation: PCNA associated factor Antibody [NBP2-32117]")
Immunoprecipitation: PCNA associated factor Antibody [NBP2-32117]
Immunoprecipitation: PCNA associated factor Antibody [NBP2-32117] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-PAF antibody NBP2-32117 used for IP at 6 ug per reaction. PAF was also immunoprecipitated by rabbit anti-PAF antibody NBP2-32117. For blotting immunoprecipitated PAF, NBP2-32117 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for PCNA associated factor Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Immunoprecipitation
2 - 10 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PCNA associated factor
Alternate Names
HCV NS5A-transactivated protein 9, Hepatitis C virus NS5A-transactivated protein 9, KIAA0101, NS5ATP9p15PAF, OEATC-1OEATC1, Overexpressed in anaplastic thyroid carcinoma 1, p15(PAF), PAF, PCNA-associated factor
Gene Symbol
PCLAF
UniProt
Additional PCNA associated factor Products
Product Documents for PCNA associated factor Antibody - BSA Free
Product Specific Notices for PCNA associated factor Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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