Patched 1/PTCH Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2451
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
This Patched 1/PTCH Antibody was developed against a peptide with sequence ELQDVECEERPR corresponding to C-Terminus according to NP_000255.1, NP_001077072.1, NP_001077071.1, NP_001077073.1, NP_001077074.1, NP_001077075.1, NP_001077076.1.
Specificity
This antibody is expected to recognise all reported isoforms. Variants (NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1) encode the same isoform.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Patched 1/PTCH Antibody
Immunocytochemistry/ Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451]
Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NB100-2451]
Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NB100-2451] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.Immunocytochemistry/ Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451]
Immunocytochemistry/Immunofluorescence: Patched 1/PTCH Antibody [NB100-2451] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Applications for Patched 1/PTCH Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Immunohistochemistry
8 ug/mL
Immunohistochemistry-Paraffin
8 ug/mL
Peptide ELISA
Detection limit 1:16000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Patched 1/PTCH
Alternate Names
BCNS, HPE7, mes, NBCCS, ptc1, PTCH1
Entrez Gene IDs
5727 (Human)
Gene Symbol
PTCH1
UniProt
Additional Patched 1/PTCH Products
Product Documents for Patched 1/PTCH Antibody
Product Specific Notices for Patched 1/PTCH Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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