Park7/DJ-1 Antibody (23GB1955)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50960
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB1955
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human Park7/DJ-1 (Uniprot: Q99497)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Park7/DJ-1 Antibody (23GB1955)
Immunocytochemistry/ Immunofluorescence: Park7/DJ-1 Antibody (23GB1955)[NBP3-50960] -
Immunocytochemical staining of HepG2 cells with Park7/DJ-1 antibody (1:1,000). Nuclei were stained blue with DAPI; Parkinsonism associated deglycase was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Flow Cytometry: Park7/DJ-1 Antibody (23GB1955)[NBP3-50960] -
Flow cytometric analysis of Park7/DJ-1expression in HepG2 cells using Park7/DJ-1 antibody (1:2,000). Green, isotype control; red, Parkinsonism associated deglycase.
Western Blot: Park7/DJ-1 Antibody (23GB1955)[NBP3-50960] -
Western blotting analysis using Park7/DJ-1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.Applications for Park7/DJ-1 Antibody (23GB1955)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Park7/DJ-1
PARK7 is highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart, and is detected at slightly lower levels in placenta and brain. The protein is also detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa.
Defects in this gene cause the autosomal recessive neurodegenerative disorder early-onset Parkinson's disease 7.
Long Name
Parkinson Disease 7
Alternate Names
DJ-1, DJ1
Gene Symbol
PARK7
Additional Park7/DJ-1 Products
Product Documents for Park7/DJ-1 Antibody (23GB1955)
Product Specific Notices for Park7/DJ-1 Antibody (23GB1955)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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