PAR/pADPr Antibody (10H)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89039
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Drosophila
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Clone # 10H
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Purified poly(ADP-ribose).
Specificity
Recognizes poly(ADP-ribose) synthesized by a broad range of PARPs (poly(ADP-ribose) polymerases) like human, mouse, rat or Drosophila PARP enzyme.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Description
Protein A-affinity purified from supernatant.
Scientific Data Images for PAR/pADPr Antibody (10H)
![Immunocytochemistry/ Immunofluorescence: PAR/pADPr Antibody (10H) [NBP2-89039]](https://resources.bio-techne.com/images/products/PAR-pADPr-Antibody-10H-Immunocytochemistry-Immunofluorescence-NBP2-89039-img0002.jpg "Immunocytochemistry/ Immunofluorescence: PAR/pADPr Antibody (10H) [NBP2-89039]")
Immunocytochemistry/ Immunofluorescence: PAR/pADPr Antibody (10H) [NBP2-89039]
Immunocytochemistry/Immunofluorescence: PAR/pADPr Antibody (10H) [NBP2-89039] - Detection of DNA damage.![Immunocytochemistry/ Immunofluorescence: PAR/pADPr Antibody (10H) [NBP2-89039]](https://resources.bio-techne.com/images/products/PAR-pADPr-Antibody-10H-Immunocytochemistry-Immunofluorescence-NBP2-89039-img0001.jpg "Immunocytochemistry/ Immunofluorescence: PAR/pADPr Antibody (10H) [NBP2-89039]")
Immunocytochemistry/ Immunofluorescence: PAR/pADPr Antibody (10H) [NBP2-89039]
Immunocytochemistry/Immunofluorescence: PAR/pADPr Antibody (10H) [NBP2-89039] - Detection of apoptotic cells by immunocytochemistryApplications for PAR/pADPr Antibody (10H)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
5-20 ug/ml
Immunohistochemistry-Paraffin
5-20 ug/ml
Application Notes
Immunohistochemistry (paraffin sections): dilution buffer: 5% milk (non fat dried milk) in PBS to a final concentration of 5-20 ug/ml). Western Blot: incubate 2.5 ug/ml in PBS, 0.05% Tween20, 5% milk (non fat dried milk)).
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50mM HEPES, pH 7.4, containing 100mM sodium chloride, 1% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PAR/pADPr
PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.
Long Name
Poly [ADP-ribose] Polymer
Alternate Names
PAR
Additional PAR/pADPr Products
Product Specific Notices for PAR/pADPr Antibody (10H)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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