PAF1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-274
![Immunoprecipitation: PAF1 Antibody [NB600-274]](https://resources.bio-techne.com/images/products/PAF1-Antibody-Immunoprecipitation-NB600-274-img0011.jpg "Immunoprecipitation: PAF1 Antibody [NB600-274]")
![Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274]](https://resources.bio-techne.com/images/products/PAF1-Antibody-Immunohistochemistry-Paraffin-NB600-274-img0010.jpg "Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide which maps to a region between residues 475 and 525 of human RNA Polymerase Associated Factor using the numbering given in TrEMBL entry Q9NUU9 (GeneID 54623).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit PAF1 Antibody - BSA Free (NB600-274) is a polyclonal antibody validated for use in IHC and IP. Anti-PAF1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for PAF1 Antibody - BSA Free
Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274]
Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti- Paf1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274]](https://resources.bio-techne.com/images/products/PAF1-Antibody-Immunohistochemistry-Paraffin-NB600-274-img0009.jpg "Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274]")
Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274]
Immunohistochemistry-Paraffin: PAF1 Antibody [NB600-274] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Paf1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Immunoprecipitation: PAF1 Antibody [NB600-274]](https://resources.bio-techne.com/images/products/PAF1-Antibody-Immunoprecipitation-NB600-274-img0008.jpg "Immunoprecipitation: PAF1 Antibody [NB600-274]")
Immunoprecipitation: PAF1 Antibody [NB600-274]
Immunoprecipitation: PAF1 Antibody [NB600-274] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Paf1 antibody NB600-274 Lot 2 used for IP at 3 ug/mg lysate. Paf1 was also immunoprecipitated by rabbit anti-Paf1 Lot 1 of the same product. For blotting immunoprecipitated Paf1, NB600-274 Lot 2 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time 30 seconds.Applications for PAF1 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500-1:2000
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
3-10 ug/mg lysate
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. For better Western Blot antibody see NB 600-273 instead. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PAF1
Alternate Names
F23149_1, FLJ11123, Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae), Pancreatic differentiation protein 2, PD2hPAF1, RNA polymerase II-associated factor 1 homolog
Entrez Gene IDs
54623 (Human)
Gene Symbol
PAF1
UniProt
Additional PAF1 Products
Product Documents for PAF1 Antibody - BSA Free
Product Specific Notices for PAF1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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