p73 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-420
![Western Blot: p73 Antibody [NB100-420]](https://resources.bio-techne.com/images/products/p73-Antibody-Western-Blot-NB100-420-img0003.jpg "Western Blot: p73 Antibody [NB100-420]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide mapping to a region between residues 1 and 62 of human tumor protein p73 using the numbering given in Swiss-Prot entry O15350 (GeneID 7161).
Specificity
The epitope is found in isoforms alpha, beta, gamma, delta, epsilon, zeta, and kappa of human p73. The antibody is not expected to react with deltaNp73.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit p73 Antibody - BSA Free (NB100-420) is a polyclonal antibody validated for use in WB and IP. Anti-p73 Antibody: Cited in 13 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for p73 Antibody - BSA Free
Western Blot: p73 Antibody [NB100-420]
Western Blot: p73 Antibody [NB100-420] - Whole cell lysate (50 ug) from HEK293T, HeLa, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p73 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.![Western Blot: p73 Antibody [NB100-420]](https://resources.bio-techne.com/images/products/p73-Antibody-Western-Blot-NB100-420-img0002.jpg "Western Blot: p73 Antibody [NB100-420]")
Western Blot: p73 Antibody [NB100-420]
Western Blot: p73 Antibody [NB100-420] - Detection of Human p73 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-p73 antibody NB100-420 used for WB at 1 ug/ml (A and B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 30 seconds (B).Applications for p73 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg of lysate
Western Blot
1:2000 - 1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: p73
Long Name
Tumor Protein p73
Alternate Names
TP73
Entrez Gene IDs
7161 (Human)
Gene Symbol
TP73
UniProt
Additional p73 Products
Product Documents for p73 Antibody - BSA Free
Product Specific Notices for p73 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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