p70 S6 Kinase/S6K [p Thr389/412] Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1049

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NB600-1049-50ug
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all p70 S6 Kinase Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide surrounding amino acids Thr389/412 of human p70 S6 Kinase/S6K

Modification

p Thr389/412

Specificity

p70 S6 Kinase/S6K [p Thr389/412] Antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at Threonine 389/412

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab2571)

Scientific Data Images for p70 S6 Kinase/S6K [p Thr389/412] Antibody - BSA Free

Western Blot: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049]

Western Blot: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049]

p70-S6-Kinase-S6K-[p-Thr389-412]-Antibody-Western-Blot-NB600-1049-img0005.jpg
Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049]

Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049]

Immunocytochemistry/Immunofluorescence: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Analysis of Phospho-p70 S6 Kinase in 293 cells.
Immunohistochemistry: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049]

Immunohistochemistry: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049]

Immunohistochemistry: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Human liver cancer tissue sections
View 2 more images

Applications for p70 S6 Kinase/S6K [p Thr389/412] Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Application Notes
Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID:32339950).

Reviewed Applications

Read 1 review rated 5 using NB600-1049 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150 mM EDTA, 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: p70 S6 Kinase

p70 S6 Kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. P70 S6 Kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5 prime oligopyrimidine tract mRNAs. A second isoform, p85 S6 Kinase, is derived from the same gene and is identical to p70 S6 Kinase except for 23 extra residues at the N-terminus that encode a nuclear localizing signal.

Alternate Names

p70-alpha, RPS6KB1, S6K1, STK14A

Gene Symbol

RPS6KB1

UniProt

Q9UBS0, P23443 (Human), NP_003152 (Human), Q8BSK8 (Mouse), NP_001107806.1 (Mouse), P67999 (Rat), NP_114191.1 (Rat)

Additional p70 S6 Kinase Products

Product Documents for p70 S6 Kinase/S6K [p Thr389/412] Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for p70 S6 Kinase/S6K [p Thr389/412] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.
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