p53 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB200-171
![Western Blot: p53 Antibody [NB200-171]](https://resources.bio-techne.com/images/products/p53-Antibody-Western-Blot-NB200-171-img0076.jpg "Western Blot: p53 Antibody [NB200-171]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown, Independent Antibodies
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation, Chromatin Immunoprecipitation Sequencing, Knockdown Validated
Cited:
Western Blot, Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residues 50-100 of human tumor protein p53 using the SwissProt entry P04637 (GeneID 7157).
Localization
Cytoplasmic and nuclear
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-305, NB100-322, NBP1-19098, NBP1-19097, NB100-376, NB100-1803, NB100-224, NB100-61615, NB100-77315, NB500-160, NB100-381, NB100-381, NB100-596, NB200-347, NB100-97835, NBP1-06527, NBP1-06515
Scientific Data Images for p53 Antibody - BSA Free
Western Blot: p53 Antibody [NB200-171]
Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (5, 15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiP53 antibody used for WB at0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunohistochemistry-Paraffin: p53 Antibody [NB200-171]](https://resources.bio-techne.com/images/products/p53-Antibody-Immunohistochemistry-Paraffin-NB200-171-img0075.jpg "Immunohistochemistry-Paraffin: p53 Antibody [NB200-171]")
Immunohistochemistry-Paraffin: p53 Antibody [NB200-171]
Immunohistochemistry-Paraffin: p53 Antibody [NB200-171] - FFPE section of human lung cancer Antibody: Affinity purified rabbit anti- p53 used at 1:5,000 (0.2ug/ml). Detection: DAB![Flow Cytometry: p53 Antibody [NB200-171]](https://resources.bio-techne.com/images/products/p53-Antibody-Flow-Cytometry-NB200-171-img0002.jpg "Flow Cytometry: p53 Antibody [NB200-171]")
Flow Cytometry: p53 Antibody [NB200-171]
Flow Cytometry: p53 Antibody [NB200-171] - p53 in Jurkat Cells, fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 million cells were stained with 1.5 ug anti-KLH IgG control or anti-p53 NB200-171 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- anti-KLH control IgG; Red- anti-HDMX.![Western Blot: p53 Antibody [NB200-171]](https://resources.bio-techne.com/images/products/p53-Antibody-Western-Blot-NB200-171-img0003.jpg "Western Blot: p53 Antibody [NB200-171]")
![Flow Cytometry: p53 Antibody [NB200-171]](https://resources.bio-techne.com/images/products/p53-Antibody-Flow-Cytometry-NB200-171-img0004.jpg "Flow Cytometry: p53 Antibody [NB200-171]")
![Immunoprecipitation: p53 Antibody [NB200-171]](https://resources.bio-techne.com/images/products/p53-Antibody-Immunoprecipitation-NB200-171-img0077.jpg "Immunoprecipitation: p53 Antibody [NB200-171]")
Applications for p53 Antibody - BSA Free
Application
Recommended Usage
Flow Cytometry
1.5 ug/10^6 cells in 150 ul
Immunohistochemistry
1:1000 - 1:5000
Immunohistochemistry-Paraffin
1:1000 - 1:5000
Immunoprecipitation
2 - 5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ChIP-Seq 4 ug/30ug chromatin.
Reviewed Applications
Read 1 review rated 5 using NB200-171 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: p53
Additional p53 Products
Product Documents for p53 Antibody - BSA Free
Product Specific Notices for p53 Antibody - BSA Free
This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-305, NB100-322, NBP1-19098, NBP1-19097, NB100-376, NB100-1803, NB100-224, NB100-61615, NB100-77315, NB500-160, NB100-381, NB100-381, NB100-596, NB200-347, NB100-97835, NBP1-06527, NBP1-06515
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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