P4HA2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06468
![Western Blot: P4HA2 Antibody [NBP3-06468]](https://resources.bio-techne.com/images/products/P4HA2-Antibody-Western-Blot-NBP3-06468-img0001.jpg "Western Blot: P4HA2 Antibody [NBP3-06468]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human P4HA2 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for P4HA2 Antibody - BSA Free
Western Blot: P4HA2 Antibody [NBP3-06468]
Western Blot: P4HA2 Antibody [NBP3-06468] - Lane A: HeLa Whole Cell LysateLane B: H1299 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: PC12 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:61 kDaObserved band size:61 kDa(We are unsure as to the identity of these extra bands.)![Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP3-06468]](https://resources.bio-techne.com/images/products/P4HA2-Antibody-Immunohistochemistry-Paraffin-NBP3-06468-img0002.jpg "Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP3-06468]")
Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP3-06468]
Immunohistochemistry-Paraffin: P4HA2 Antibody [NBP3-06468] - Staining of human P4HA2 in human epididymis with rabbit polyclonal antibody at 1:2000 dilution.![Immunoprecipitation: P4HA2 Antibody [NBP3-06468]](https://resources.bio-techne.com/images/products/P4HA2-Antibody-Immunoprecipitation-NBP3-06468-img0004.jpg "Immunoprecipitation: P4HA2 Antibody [NBP3-06468]")
Immunoprecipitation: P4HA2 Antibody [NBP3-06468]
Immunoprecipitation: P4HA2 Antibody [NBP3-06468] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-P4HA2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-P4HA2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 61 kDaObserved band size :61 kDaApplications for P4HA2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry-Paraffin
1:500-1:5000
Immunoprecipitation
1-5uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Format
BSA Free
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: P4HA2
Alternate Names
2-oxoglutarate-4-dioxygenase subunit alpha-2, 4-PH alpha 2, 4-PH alpha-2, alpha polypeptide II, collagen prolyl 4-hydroxylase alpha(II), C-P4Halpha(II), EC 1.14.11.2, Procollagen-proline, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), prolyl 4-hydroxylase subunit alpha-2, prolyl 4-hydroxylase, alpha polypeptide II
Gene Symbol
P4HA2
Additional P4HA2 Products
Product Specific Notices for P4HA2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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