P2Y2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15060
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human P2Y2. The exact sequence is proprietary.
Reactivity Notes
Pig (82%), Bovine (82%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit P2Y2 Antibody - BSA Free (NBP2-15060) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for P2Y2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: P2Y2 Antibody [NBP2-15060] -
Immunocytochemistry/ Immunofluorescence: P2Y2 Antibody [NBP2-15060] - P2Y2 antibody [N3C3] detects P2Y2 protein at cell membrane by immunofluorescent analysis.Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: P2Y2 stained by P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:500.Blue: Hoechst 33342 staining.Western Blot: P2Y2 Antibody [NBP2-15060] -
Western Blot: P2Y2 Antibody [NBP2-15060] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: P2Y2 Antibody [NBP2-15060] -
Boiled and unboiled HL-60 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)Applications for P2Y2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS
Format
BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: P2Y2/P2RY2
Long Name
P2Y Purinergic Receptor 2
Alternate Names
P2RU1, P2RY2, P2U, P2U1, P2Y2R
Gene Symbol
P2RY2
UniProt
Additional P2Y2/P2RY2 Products
Product Documents for P2Y2 Antibody - BSA Free
Product Specific Notices for P2Y2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.