p27/Kip1 Antibody (23GB1825)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52003
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB1825
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human p27/Kip1 (Uniprot: P46527)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for p27/Kip1 Antibody (23GB1825)
Immunocytochemistry/ Immunofluorescence: p27/Kip1 Antibody (23GB1825)[NBP3-52003] -
Immunocytochemical staining of HAP-1 cells with p27/Kip1 antibody (1:1,000). Nuclei were stained blue with DAPI; p27/Kip1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Flow Cytometry: p27/Kip1 Antibody (23GB1825)[NBP3-52003] -
Validation of p27/Kip1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HSHC cells were stained with p27/Kip1 antibody (1:2,000) and analyzed using BD flow cytometer.
Flow Cytometry: p27/Kip1 Antibody (23GB1825)[NBP3-52003] -
Flow cytometric analysis of p27/Kip1expression in HAP-1 cells using p27/Kip1 antibody (1:2,000). Green, isotype control; red, Cyclin dependent kinase inhibitor 1B.Applications for p27/Kip1 Antibody (23GB1825)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: p27/Kip1
Long Name
p27 Cyclin Dependent Kinase 4 Inhibitor 1B
Alternate Names
CDKN1B, Kip1
Gene Symbol
CDKN1B
Additional p27/Kip1 Products
Product Documents for p27/Kip1 Antibody (23GB1825)
Product Specific Notices for p27/Kip1 Antibody (23GB1825)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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